An E3 trailer for No More Heroes: Desperate Struggle has been put on YouTube. Travis Touchdown and Sylvia both make a comeback, along with a host of new characters driven by a revenge-fueled storyline.
There's also an indication that NMH:DS will support MotionPlus, which would make sense for the combat aspects. This time around, Travis has the ability to wield two Katana beams at once. The game is still on track for a 2010 release.